Identification and characterization of single-domain thiosulfate sulfurtransferases from Arabidopsis thaliana.
Sulfurtransferases/rhodaneses ( ST) are a group of enzymes widely distributed in all three phyla that catalyze the transfer of sulfur from a donor to a thiophilic acceptor substrate. All ST contain distinct structural domains, and can exist as single-domain proteins, as tandemly repeated modules in which the C-terminal domain bears the active site, or as members of multi-domain proteins. We identified several ST in Arabidopsis resembling the C-terminus of the Arabidopsis two-domain ST1 and the single-domain GlpE protein from Escherichia coli. Two of them (accession numbers BAB10422 and BAB10409) were expressed in E. coli and purified. Both proteins showed thiosulfate-specific ST enzyme activity.[1]References
- Identification and characterization of single-domain thiosulfate sulfurtransferases from Arabidopsis thaliana. Bauer, M., Papenbrock, J. FEBS Lett. (2002) [Pubmed]
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