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Crystallization and preliminary X-ray crystallographic analysis of Sclerotium rolfsii lectin.

Sclerotium rolfsii lectin (SRL), from the soil-borne phytopathogenic fungus S. rolfsii, has been crystallized. SRL crystals were grown by the hanging-drop vapour-diffusion method using an MPD-ammonium acetate mixture in Tris-HCl buffer pH 8. 5. A complete data set from a single crystal at 100 K was collected to 1.1 A resolution using synchrotron radiation. Preliminary crystallographic analysis showed that the crystals belong to the tetragonal space group P4(2)2(1)2, with unit-cell parameters a = b = 99.81, c = 63.99 A and two molecules per asymmetric unit.[1]

References

  1. Crystallization and preliminary X-ray crystallographic analysis of Sclerotium rolfsii lectin. Leonidas, D.D., Swamy, B.M., Bhat, A.G., Inamdar, S.R., Kosmopoulou, M.N., Chrysina, E.D., Oikonomakos, N.G. Acta Crystallogr. D Biol. Crystallogr. (2003) [Pubmed]
 
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