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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

A sensitive bioassay for chicken interleukin-18 based on the inducible release of preformed interferon-gamma.

Conventional tests for measuring the biological activity of chicken interleukin (IL)-18 require primary chicken spleen cells. We now describe a sensitive bioassay that is based on interleukin-18- induced release of interferon (IFN)-gamma by a permanent chicken cell line. In B19-2D8 cells, cytoplasmically stored interferon-gamma is quickly secreted in response to interleukin-18 exposure.[1]

References

  1. A sensitive bioassay for chicken interleukin-18 based on the inducible release of preformed interferon-gamma. Puehler, F., Göbel, T., Breyer, U., Ohnemus, A., Staeheli, P., Kaspers, B. J. Immunol. Methods (2003) [Pubmed]
 
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