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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

A fluorogenic dye activated by the staudinger ligation.

Specific labeling of biomolecules with biochemical and biophysical probes is a central element of proteomics research. Here we describe a coumarin-phosphine dye that undergoes activation of coumarin fluorescence upon Staudinger ligation with azides. Since azides can be metabolically incorporated into cellular proteins and oligosaccharides, this dye may be a useful tool for profiling proteins and their posttranslational modifications.[1]


  1. A fluorogenic dye activated by the staudinger ligation. Lemieux, G.A., De Graffenried, C.L., Bertozzi, C.R. J. Am. Chem. Soc. (2003) [Pubmed]
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