Molecular cloning and characterization of the Delta9 fatty acid desaturase gene and its promoter region from Saccharomyces kluyveri.
A gene encoding Delta9 fatty acid desaturase, which catalyzes the first reaction of fatty acid desaturation, has been cloned from Saccharomyces kluyveri, a species capable of producing polyunsaturated fatty acids (poly-UFA). The open reading frame of this gene (named Sk-OLE1) consists of 1443 bp, encoding 480 amino acids. The deduced Sk-OLE1 protein shared 63-74% identity with those from other yeasts. Expression of the Sk-OLE1 gene in Saccharomyces cerevisiae complemented genetic disruption of the S. cerevisiae OLE1 gene (Sc-OLE1). Fatty acid analysis showed that the ratio of palmitoleic acid to oleic acid in the Sk-OLE1-expressing S. cerevisiae strain was lower than that of the S. cerevisiae strain expressing the Sc-OLE1 gene. The promoter region of the Sk-OLE1 gene was shorter than that of the Sc-OLE1 gene and appeared to lack the fatty acid-regulated sequences present in the Sc-OLE1 promoter region. Analysis of transcriptional expression revealed that the Sk-OLE1 gene was not actually repressed in either S. kluyveri or S. cerevisiae by exogenous UFAs. The DDBJ accession number for the sequence reported in this paper is AB071696.[1]References
- Molecular cloning and characterization of the Delta9 fatty acid desaturase gene and its promoter region from Saccharomyces kluyveri. Kajiwara, S. FEMS Yeast Res. (2002) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
