Airway obstruction after acute ozone exposure in BALB/c mice using barometric plethysmography

Korean J Intern Med. 2003 Mar;18(1):1-5. doi: 10.3904/kjim.2003.18.1.1.

Abstract

Background: Airway responsiveness after acute inhalation of ozone is related to the concentration and duration of ozone exposure. Using barometric whole-body plethysmography and increase in enhanced pause (Penh) as an index of airway obstruction, we measured the response of BALB/c mice to acute ozone inhalation to study the time course change of pulmonary function after ozone exposure.

Methods: Penh was measured before and after exposure to filtered air or 0.12, 0.5, 1, or 2 ppm ozone for 3 hr (n = 6/group). In addition, Penh was measured 24, 48 and 72 hr after ozone exposure. Bronchoalveolar lavage (BAL) and histopathologic examinations were performed.

Results: The increase in Penh after ozone exposure was significantly higher in the 0.12, 0.5, 1 and 2 ppm groups compared with the control group (all p < 0.01). Increases in Penh 24 hr after ozone exposure were significantly lower than those immediately after acute ozone exposure; however, increases in Penh 72 hr after ozone exposure were significantly higher than those in the control group (each p < 0.01). The proportion of neutrophils in BAL fluid was significantly higher in the group exposed to 2 ppm ozone than in the groups exposed to filtered air or 0.12 ppm ozone (both p < 0.01).

Conclusion: These results indicate that airway obstruction is induced following ozone exposure in a concentration-dependent manner and persists for at least 72 hr.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Airway Obstruction / etiology*
  • Airway Obstruction / pathology*
  • Animals
  • Animals, Newborn
  • Bronchoalveolar Lavage Fluid / cytology
  • Disease Models, Animal
  • Female
  • Mice
  • Mice, Inbred BALB C
  • Plethysmography, Whole Body / methods*
  • Probability
  • Reference Values
  • Respiratory Function Tests
  • Risk Assessment
  • Sensitivity and Specificity
  • Statistics, Nonparametric
  • Sulfuric Acids / adverse effects*

Substances

  • Sulfuric Acids
  • potassium peroxymonosulfuric acid