Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos.
A novel approach for chromosome doubling that consists of treating embryos instead of parts of micropropagated plants was investigated. Following 2-year trials, amiprofos-methyl (APM) was found to be superior to oryzalin on the basis of a lower toxicity, and we were able to narrow the range of concentrations of APM. The addition of 2% dimethyl sulphoxide (DMSO) and 1% Triton X-100 to 25 microM APM had no effect in all treatments. A final experiment with 6,658 embryos demonstrated that a 2-day treatment in liquid media supplemented with 50 microM APM was the most successful with respect to chromosome doubling-36.7% of the plants were diploid-but the survival rate was reduced to 52.5% of that of the non-treated control. A 2-day treatment in liquid medium supplemented with 25 microM APM or a 2-day treatment on solid medium with 50 microM APM resulted in the production of diploids at a frequency of 28.9% and 21.3%, respectively. These may represent alternative methods for chromosome doubling since compared to the untreated control these two treatments reduced the survival rate by only about 24%. Final ploidy and fertility of the large proportion of induced mixoploid plants (up to 30.3%) need to be evaluated in further studies.[1]References
- Chromosome doubling procedures of onion (Allium cepa L.) gynogenic embryos. Jakse, M., Havey, M.J., Bohanec, B. Plant Cell Rep. (2003) [Pubmed]
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