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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Characterization of beta-adrenergic receptors in DiFi and HT-29 cells.

The beta-adrenergic receptors present in two human colorectal adenocarcinoma cell lines were characterized by measuring specific binding of [125I]-cyanopindolol ( CYP). Whole, cultured, DiFi (derived from a familial adenomatous polyposis [ FAP] patient) and HT-29 cells were used in radioligand binding assays. Scatchard analysis of specific 125I-CYP binding gave KDS of 38.6 +/- 5.7 pM in DiFi cells and 54 +/- 9.1 pM in HT-29 cells. However, binding site density (Bmax) in the DiFi cells was greater than that in HT-29 cells. In DiFi cells, the kinetically determined KD was similar to that calculated from Scatchard analysis. Studies in DiFi cells of the displacement of specific 125I-CYP binding by nonselective (propranolol), beta 1-selective (metoprolol and atenolol), and beta 2-selective (ICI 118-551) antagonists revealed only a single class of beta 2-adrenergic receptors. This provides the first evidence that colorectal adenocarcinoma cell lines contain beta-adrenergic receptors and shows that only beta 2-adrenergic receptors are present in DiFi cells. Mechanisms possibly affecting beta-adrenergic-receptor expression in such cells are discussed in relation to colon carcinogenesis.[1]


  1. Characterization of beta-adrenergic receptors in DiFi and HT-29 cells. Abel, P.W., Zeng, W., Wildrick, D.M., Makoid, M.C., Boman, B.M. Anticancer Res. (1992) [Pubmed]
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