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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A rapid and sensitive heterogeneous immunoelectrochemical assay using disposable electrodes.

In this novel enzyme-tagged immuoelectrochemical assay, disposable carbon felt discs serve both as electrodes and as the heterogeneous solid phase. Antibodies are immobilized on the carbon felt via a diaminoalkane-biotin-avidin-biotin bridge. Alkaline phosphatase is used as a label. Bound antibodies are monitored by following the electro-oxidation of aminophenol, produced enzymatically from p-amino-phenyl phosphate by the immobilized alkaline phosphatase at the electrode surface. A model system designed for determination of mouse IgG concentration yielded a calibration curve ranging from 10 pg/ml to 100 micrograms/ml. This assay can be performed rapidly and a single determination completed within 20 minutes. The system is useful also for rapid quantitation of a small number (approximately 80 organisms per ml) of bacteria.[1]

References

  1. A rapid and sensitive heterogeneous immunoelectrochemical assay using disposable electrodes. Hadas, E., Soussan, L., Rosen-Margalit, I., Farkash, A., Rishpon, J. Journal of immunoassay. (1992) [Pubmed]
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