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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Initiation on chromatin templates in a yeast RNA polymerase II transcription system.

Templates were prepared with either the TATA box or transcription start sites of the yeast CYC1 promoter in a nucleosome. In both cases, initiation in an unfractionated yeast RNA polymerase II transcription system was abolished by the nucleosome. The inhibition appeared to be relieved by the activator protein Gal4-VP16 binding to a site upstream of the promoter. Inhibition was not relieved, however, in a transcription system reconstituted from purified components, indicating a requirement for additional factors for the effect of Gal4-VP16.[1]

References

  1. Initiation on chromatin templates in a yeast RNA polymerase II transcription system. Lorch, Y., LaPointe, J.W., Kornberg, R.D. Genes Dev. (1992) [Pubmed]
 
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