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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Direct interaction between alpha-actinin and hepatitis C virus NS5B.

It has been suggested that cellular proteins are involved in hepatitis C virus (HCV) RNA replication. By using the yeast two-hybrid system, we isolated seven cDNA clones encoding proteins interacting with HCV RNA polymerase (NS5B) from a human liver cDNA library. For one of these, alpha-actinin, we confirmed the interaction by coimmunoprecipitation, immunofluorescent staining and confocal microscopic analysis. Experiments with deletion mutants showed that domains NS5B(84-95), NS5B(466-478), and alpha-actinin(621-733) are responsible for the interaction. Studies of the HCV subgenomic replicon system with small interference RNA indicate that alpha-actinin is essential for HCV RNA replication. Our results suggest alpha-actinin may be a component of the HCV replication complex.[1]

References

  1. Direct interaction between alpha-actinin and hepatitis C virus NS5B. Lan, S., Wang, H., Jiang, H., Mao, H., Liu, X., Zhang, X., Hu, Y., Xiang, L., Yuan, Z. FEBS Lett. (2003) [Pubmed]
 
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