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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Intracellular compartmentation in the biosynthesis of caulerpenyne: study on intact macroalgae using stable-isotope-labeled precursors.

The biosynthesis of caulerpenyne 1 was studied in the invasive green alga Caulerpa taxifolia. The investigation was performed on intact algae with stable-isotope-labeled precursors administered under mixotrophic growth conditions. According to the labeling pattern, after incorporation of 1-(13)C-acetate and (13)CO(2), respectively, the biosynthesis of the sesquiterpene backbone occurs in the chloroplast and follows the methyl-erythritol-4-phosphate (MEP) pathway. In contrast, the acetyl residues of caulerpenyne 1 are derived from a cytosolic resource. [structure: see text][1]

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