The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Quantitative measurements of Ca(2+)/calmodulin binding and activation of myosin light chain kinase in cells.

Myosin II regulatory light chain ( RLC) phosphorylation by Ca(2+)/calmodulin (CaM)-dependent myosin light chain kinase ( MLCK) is implicated in many cellular actin cytoskeletal functions. We examined MLCK activation quantitatively with a fluorescent biosensor MLCK where Ca(2+)-dependent increases in kinase activity were coincident with decreases in fluorescence resonance energy transfer (FRET) in vitro. In cells stably transfected with CaM sensor MLCK, increasing [Ca(2+)](i) increased MLCK activation and RLC phosphorylation coincidently. There was no evidence for CaM binding but not activating MLCK at low [Ca(2+)](i). At saturating [Ca(2+)](i) MLCK was not fully activated probably due to limited availability of cellular Ca(2+)/CaM.[1]

References

  1. Quantitative measurements of Ca(2+)/calmodulin binding and activation of myosin light chain kinase in cells. Geguchadze, R., Zhi, G., Lau, K.S., Isotani, E., Persechini, A., Kamm, K.E., Stull, J.T. FEBS Lett. (2004) [Pubmed]
 
WikiGenes - Universities