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Establishment of transgenic mouse model carring the promoter of plunc sequence.

OBJECTIVE: To construct the vector using specific nasopharynx tissue promoter plunc to establish transgeneic mouse model. METHODS: Plunc- EGFP plasmid was digested with XhoI, the purified linearized DNA fragments were recovered by gel extraction and diluted to the final concentration of 4 microg/ml, before introduced into fertilized one-cell mouse eggs by pronuclear microinjection. RESULTS: Thirteen founder mice were obtained, 12 of which were positive for the integrated EGFP gene as detected by PCR, and 3 were positive shown by Southern blotting. CONCLUSION: Specific nasopharynx tissue promoter plunc can effectively induce exogenous gene integration into the mouse genome.[1]

References

  1. Establishment of transgenic mouse model carring the promoter of plunc sequence. Yang, Y.F., Ding, Y.Q., Zhang, L., Liang, L. Di Yi Jun Yi Da Xue Xue Bao (2004) [Pubmed]
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