The plant microtubule-associated protein AtMAP65-3/ PLE is essential for cytokinetic phragmoplast function.
Directional cell expansion in interphase and nuclear and cell division in M-phase are mediated by four microtubule arrays, three of which are unique to plants: the interphase array, the preprophase band, and the phragmoplast. The plant microtubule-associated protein MAP65 has been identified as a key structural component in these arrays. The Arabidopsis genome has nine MAP65 genes, and here we show that one, AtMAP65-3/ PLE, locates only to the mitotic arrays and is essential for cytokinesis. The Arabidopsis pleiade ( ple) alleles are single recessive mutations, and we show that these mutations are in the AtMAP65-3 gene. Moreover, these mutations cause C-terminal truncations that abolish microtubule binding. In the ple mutants the anaphase spindle is normal, and the cytokinetic phragmoplast can form but is distorted; not only is it wider, but the midline, the region where oppositely oriented microtubules overlap, is unusually expanded. Here we present data that demonstrate an essential role for AtMAP65-3/ PLE in cytokinesis in plant cells.[1]References
- The plant microtubule-associated protein AtMAP65-3/PLE is essential for cytokinetic phragmoplast function. Müller, S., Smertenko, A., Wagner, V., Heinrich, M., Hussey, P.J., Hauser, M.T. Curr. Biol. (2004) [Pubmed]
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