The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Characterization of COBRA1 in human breast cancer cell lines using a new polyclonal antibody against COBRA1.

Mutations in the breast cancer susceptibility gene BRCA1 predispose individuals to breast and ovarian cancers. Cofactor of BRCA1 (COBRA1), a novel protein, was isolated as a BRCA1-interacting protein. However, the role of COBRA1 in breast cancer is poorly understood. In this study, we demonstrate that COBRA1 mRNA was differentially expressed in breast cancer cell lines by semi-quantitative reverse transcription-polymerase chain PCR (RT-PCR). We developed a highly specific rabbit polyclonal anti-COBRA1 antibody using GST-COBRA1 fusion protein. In most cases, the levels of COBRA1 protein in breast cancer cell lines detected by Western blotting with the anti-COBRA1 antibody correlated with those of COBRA1 mRNA. Immunofluorescence analysis indicated that COBRA1 was a nuclear protein. Endogenously expressed COBRA1 interacted with the nuclear protein BRCA1 in human breast cancer cells. These data suggest that the COBRA1 antibody may be a useful tool to investigate functions of COBRA1 in cancers and that, like BRCA1, COBRA1 may regulate various nuclear events in breast cancer cells.[1]

References

  1. Characterization of COBRA1 in human breast cancer cell lines using a new polyclonal antibody against COBRA1. Zhu, J., Song, S., Jiang, Z., Yan, J., Lu, Q., Huang, C., Ye, Q. IUBMB Life (2004) [Pubmed]
 
WikiGenes - Universities