Nonomuraea kuesteri sp. nov

Int J Syst Evol Microbiol. 2005 Mar;55(Pt 2):847-851. doi: 10.1099/ijs.0.63380-0.

Abstract

A Gram-positive, aerobic, non-motile actinomycete, strain GW 14-1925(T), that formed branched substrate and aerial mycelium was studied using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity studies, strain GW 14-1925(T) was shown to belong to the genus Nonomuraea, being most closely related to Nonomuraea longicatena (97.9 %), Nonomuraea turkmeniaca (98.9 %), Nonomuraea helvata (98.6 %), Nonomuraea polychroma (98.5 %), Nonomuraea salmonaea (98.3 %), Nonomuraea roseoviolacea subsp. roseoviolacea (98.1 %) and Nonomuraea roseoviolacea subsp. carminata (97.7 %). The 16S rRNA gene sequence similarity to other Nonomuraea species was <97.5 %. Chemotaxonomic data [major menaquinones of the MK-9 series with minor amounts of MK-8(H(4)); major polar lipids of phospholipid type IV; fatty acids with major amounts of iso- and anteiso- and 10-methyl-branched fatty acids in combination with iso-branched 2-hydroxy fatty acids] supported allocation of the strain to the genus Nonomuraea. The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW 14-1925(T) from closely related species; thus, GW 14-1925(T) represents a novel species of the genus Nonomuraea, for which the name Nonomuraea kuesteri sp. nov. is proposed, with GW 14-1925(T) (=DSM 44753(T)=NRRL B-24325(T)) as the type strain.

MeSH terms

  • Actinomycetales / classification*
  • Actinomycetales / genetics
  • Actinomycetales / metabolism
  • Actinomycetales / physiology
  • Bacterial Typing Techniques
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Genes, rRNA
  • Molecular Sequence Data
  • Mycelium / physiology*
  • Nucleic Acid Hybridization
  • Phenotype
  • RNA, Ribosomal, 16S / genetics
  • Soil Microbiology*

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AJ746362