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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Assignment of the human alpha 2-plasmin inhibitor gene (PLI) to chromosome 17, region pter- p12, by PCR analysis of somatic cell hybrids.

The human gene for the alpha 2-plasmin inhibitor (PLI) had been assigned by others to the pericentromeric region of chromosome 18 by in situ hybridization. However, when we used a probe for this gene in our efforts to construct a complete physical map of chromosome 18, we discovered that PLI could be excluded from this chromosome. On the basis of the published PLI sequence, we designed primers to sequences in intron 6 and 7 that direct amplification of a 353-bp fragment that includes the entire exon 7. By using PCR analysis of rodent x human hybrid panels, we have unequivocally assigned the PLI locus to human chromosome 17. With a regional mapping panel, the assignment could be narrowed to region 17pter-p12.[1]

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