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Cloning and sequencing of Indian water buffalo interleukin-18 cDNA.

Summary Full-length cDNA (582 bp) of the interleukin-18 (IL-18) gene of the Indian water buffalo (Bubalus bubalis) was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The deduced amino acid sequence has 99% and 95% similarity with the IL-18 sequences of cattle and sheep, respectively. There are two amino acid substitutions at positions 132 and 182 in buffalo IL-18 compared with that of cattle. Phylogenetic analysis showed that the IL-18 sequence of fish forms a different lineage and is most divergent from that of cattle, buffalo, sheep, pig, dog, horse, human, monkey, mouse, rat and chicken.[1]

References

  1. Cloning and sequencing of Indian water buffalo interleukin-18 cDNA. Chaudhury, P., Bera, B.C. International journal of immunogenetics. (2005) [Pubmed]
 
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