Generation of recombinant norovirus-like particles ( VLP) in the human endothelial kidney cell line 293T.
Noroviruses (NVs) are the major cause of non-bacterial outbreaks of gastroenteritis. Here we report a new alternative system to generate recombinant NV virus-like particles (rNV- VLP) in a human endothelial kidney cell line ( HEK). Transfecting HEK-293T cells with an expression vector coding for the ORF-2 gene lead to the expression of the viral structural protein VP1 which spontaneously assembled into virus-like particles ( VLP), as shown by electron microscopy. The transfected cells did not show a cytopathic effect and released rNV- VLP into the culture medium. The HEK-293T cell derived particles were morphologically indistinguishable to the rNV- VLP produced from baculovirus and the Venezuelan equine encephalitis virus (VEE)-replicon. The produced particles were stable for at least 2.5 months at 4 degrees C.[1]References
- Generation of recombinant norovirus-like particles (VLP) in the human endothelial kidney cell line 293T. Taube, S., Kurth, A., Schreier, E. Arch. Virol. (2005) [Pubmed]
Annotations and hyperlinks in this abstract are from individual authors of WikiGenes or automatically generated by the WikiGenes Data Mining Engine. The abstract is from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenesOpen Access LicencePrivacy PolicyTerms of Useapsburg
