Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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von Lilienfeld-Toal, M. et al.

von Lilienfeld-Toal, Sievers, Bodemüller, Mihailescu, Märten, Gorschlüter, Schmidt-Wolf,

Coculture with dendritic cells promotes proliferation but not cytotoxic activity of gamma/delta T cells.

T cells bearing the gamma9/delta2 T cell receptor (TCR) have recently raised interest as non-MHC restricted effector cells against multiple myeloma. They are described to be stimulated by phosphoantigens without the need of antigen presenting cells. However, in the past a positive effect of cells of the monocyte lineage on activation of gamma/delta T cells has been shown. Monocyte derived dendritic cells (DC) are professional antigen presenting cells widely investigated as stimulators of alpha/beta T cells. But only little is known about the interaction of gamma/delta T cells and monocyte derived DC. Here, we investigated the effect of coculture of mature DC unpulsed or pulsed with ibandronate on the proliferation and cytotoxic activity of isolated gamma/delta T cells. After coculturing monocyte derived DC with isolated gamma/delta T cells, proliferation of gamma/delta T cells was enhanced as determined by the (3)H thymidine uptake assay. Also, IFN-gamma secretion was increased after coculture with DC. As DC are well known to induce activation of alpha/beta T cells we investigated whether the cytotoxic activity of gamma/delta T cells could be increased by coculture with DC. We found no difference in cytotoxic activity of gamma/delta T cells alone or cocultured with unpulsed or pulsed mature DC. Also, sensitizing of myeloma cells by addition of ibandronate could not increase lysis by gamma/delta T cells. In conclusion, monocyte derived DC are capable of stimulating proliferation and secretion of IFN-gamma of gamma/delta T cells but do not exert an effect on cytotoxic activity of gamma/delta T cells against myeloma cells.[1]

References

Coculture with dendritic cells promotes proliferation but not cytotoxic activity of gamma/delta T cells. von Lilienfeld-Toal, M., Sievers, E., Bodemüller, V., Mihailescu, C., Märten, A., Gorschlüter, M., Schmidt-Wolf, I.G. Immunol. Lett. (2005) [Pubmed]

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