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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Use of chlorite to improve HPLC detection of pyridoxal 5'-phosphate.

The sensitivity of fluorescent detection of the biologically active form of Vitamin B-6, pyridoxal 5'-phosphate ( PLP), in biological samples has been improved approximately four-fold by adopting chlorite as a post-column derivatization reagent (instead of bisulfite) in high-performance liquid chromatography (HPLC) separation. Chlorite oxidizes PLP to the more fluorescent 4-pyridoxic acid 5'-phosphate, and avoids the toxicity and heating of the cyanide procedure. Detection of another major metabolite, 4-pyridoxic acid (4-PA), is not effected. Detection of pyridoxal (PL) is slightly lowered due to eluting at a lower pH.[1]

References

  1. Use of chlorite to improve HPLC detection of pyridoxal 5'-phosphate. Ericson, K.L., Mahuren, J.D., Zubovic, Y.M., Coburn, S.P. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. (2005) [Pubmed]
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