The protective immune response induced by B cell epitope of classical swine fever virus glycoprotein E2.
Classical swine fever virus (CSFV) envelope glycoprotein E2 is a major protective immunogen responsible for eliciting neutralizing antibodies and conferring protective immunity against the virus. Based on the core sequence (TAVSPTTLR, 829-837 aa) of the B cell linear epitope of the CSFV E2 protein identified by Lin et al. [Lin, M., Lin, F., Mallory, M., Alfonso, C., 2004. Deletions of structural glycoprotein E2 of classical swine fever virus strain Alfort/187Resolve a lineal epitope of monoclonal antibody WH303 and the minimal N-terminal domain essential for binding immunoglobulin G antibodies of a pig hyperimmune serum. J. Virol. 74 (24), 11619-11625], two oligonucleotides MF and MR were synthesized and used to construct by PCR a gene cassette encoding a 15 amino acid polypeptide M (CTAVSPTTLRTEVVK), which spans 828-842 amino acids of E2. The gene cassette was fused in-frame to 3' terminal of glutathione S transferase gene ( GST) of the prokaryotic expression vector pGEX-6p-1, resulting in the recombinant plasmid pGEX-M. After transformation into Escherichia coli BL21 a soluble fusion protein GST-M with expected size of 28kDa was expressed after inducing with isopropyl-beta-d-thiogalactoside (IPTG). Enzyme-linked immunosorbent assay (ELISA) and Western blot analysis showed that the purified GST-M had good reactivity with swine anti-CSFV serum and rabbit anti-CSFV E2 serum. Further vaccination trials showed that the fusion protein GST-M could elicit effectively immune response protecting rabbits and pigs from virulent challenge. This study showed a possibility for developing epitope-based vaccines against CSFV.[1]References
- The protective immune response induced by B cell epitope of classical swine fever virus glycoprotein E2. Liu, S., Tu, C., Wang, C., Yu, X., Wu, J., Guo, S., Shao, M., Gong, Q., Zhu, Q., Kong, X. J. Virol. Methods (2006) [Pubmed]
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