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Indirect competitive immunoassay for detection of aflatoxin B(1) in corn and nut products using the array biosensor.

Because of the potential health risks of aflatoxin B(1) (AFB(1)), it is essential to monitor the level of this mycotoxin in a variety of foods. An indirect competitive immunoassay has been developed using the NRL array biosensor, offering rapid, sensitive detection and quantification of AFB(1) in buffer, corn and nut products. AFB(1)-spiked foods were extracted with methanol and Cy5-anti-AFB(1) added to the resulting sample. The extracted sample/antibody mix was passed over a waveguide surface patterned with immobilized AFB(1). The resulting fluorescence signal decreased as the concentration of AFB(1) in the sample increased. The limit of detection for AFB(1) in buffer, 0.3ng/ml, was found to increase to between 1.5 and 5.1ng/g and 0.6 and 1.4ng/g when measured in various corn and nut products, respectively.[1]

References

  1. Indirect competitive immunoassay for detection of aflatoxin B(1) in corn and nut products using the array biosensor. Sapsford, K.E., Taitt, C.R., Fertig, S., Moore, M.H., Lassman, M.E., Maragos, C.M., Shriver-Lake, L.C. Biosensors & bioelectronics. (2006) [Pubmed]
 
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