IR-MALDI-LDI combined with ion mobility orthogonal time-of-flight mass spectrometry

J Proteome Res. 2006 Jun;5(6):1484-7. doi: 10.1021/pr060055l.

Abstract

Most MALDI instrumentation uses UV lasers. We have designed a MALDI-IM-oTOF-MS which employs both a Nd:YAG laser pumped optical parametric oscillator (OPOTEK, lambda = 2.8-3.2 microm at 20 Hz) to perform IR-LDI or IR-MALDI and a Nd:YLF laser (Crystalaser, lambda = 249 nm at 200 Hz) for the UV. Ion mobility (IM) gives a fast separation and analysis of biomolecules from complex mixtures in which ions of similar chemical type fall along well-defined "trend lines". Our data shows that ion mobility allows multiply charged monomers and multimers to be resolved; thus, yielding pure spectra of the singly charged protein ion which are virtually devoid of chemical noise. In addition, we have demonstrated that IR-LDI produced similar results as IR-MALDI for the direct tissue analysis of phospholipids from rat brain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism
  • Chick Embryo
  • Cytochromes c / analysis
  • Horses
  • Lasers
  • Mass Spectrometry / methods*
  • Muramidase / analysis
  • Myocardium / enzymology
  • Phospholipids / analysis*
  • Proteins / analysis*
  • Rats
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Ultraviolet Rays

Substances

  • Phospholipids
  • Proteins
  • Cytochromes c
  • Muramidase