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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Whole Genome Phage Display Selects for Proline-rich Boi Polypeptides against Bem1p.

Interaction selection by biopanning from a fragmented yeast proteome displayed on filamentous phage particles was successful in identifying proline-rich fragments of Boi1p and Boi2p. These proteins bind to the second ;;src homology region 3'' (SH3) domain of Bem1p, a protein of Saccharomyces cerevisiae involved in bud formation. Target Bem1p was a doubly-tagged recombinant, Bem1([Asn142-Ile551]), which strongly interacts in ELISA with a C-terminal 75 amino acids polypeptide from Cdc24p exposed on phage. The whole yeast genomic display library contained ~7.7 x 10(7) independent clones of sheared S. cerevisiae genomic DNA fused to a truncated M13 gene III. This study corroborates the value of fragmented-proteome display to identify strong and direct interacting protein modules.[1]

References

  1. Whole Genome Phage Display Selects for Proline-rich Boi Polypeptides against Bem1p. Hertveldt, K., Robben, J., Volckaert, G. Biotechnol. Lett. (2006) [Pubmed]
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