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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Sancho, A.I. et al.

Sancho, Foxall, Browne, Dey, Zuidmeer, Marzban, Waldron, van Ree, Hoffmann-Sommergruber, Laimer, Mills,

Effect of postharvest storage on the expression of the apple allergen Mal d 1.

Consumption of fresh apples can cause allergy in susceptible individuals. A competitive enzyme-linked immunosorbent assay (ELISA) has been developed to determine Mal d 1 levels in apple pulp using a monoclonal antibody (BIP-1). The ELISA was able to rank ten cultivars according to their Mal d 1 content (between 3.8 and 72.5 mug/g pulp). For the first time, it has been demonstrated that growing conditions and postharvest storage, using three different treatments over a 5 month period in 2 consecutive years, increase Mal d 1 expression at a translational and transcriptional level (3.5- and 8.5-fold under controlled atmosphere storage). Expression of three major Mal d 1 isoforms was observed by real-time polymerase chain reaction over the 5 month storage period, and Mal d 1.02 was the most highly expressed isoform. In conclusion, Mal d 1 gene expression was significantly increased during modified atmosphere storage. Individuals suffering from birch pollen-apple allergy syndrome might experience fewer problems consuming freshly picked apples.[1]

References

Effect of postharvest storage on the expression of the apple allergen Mal d 1. Sancho, A.I., Foxall, R., Browne, T., Dey, R., Zuidmeer, L., Marzban, G., Waldron, K.W., van Ree, R., Hoffmann-Sommergruber, K., Laimer, M., Mills, E.N. J. Agric. Food Chem. (2006) [Pubmed]

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