Engineering RNA sequence specificity of Pumilio repeats.
Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A "code" for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here, we report the creation of seven soluble mutant proteins with predictably altered sequence specificity, including one that binds tightly to adenosine-uracil-rich element RNA. These data show that Pumilio1 can be used as a scaffold to engineer RNA-binding proteins with designed sequence specificity.[1]References
- Engineering RNA sequence specificity of Pumilio repeats. Cheong, C.G., Hall, T.M. Proc. Natl. Acad. Sci. U.S.A. (2006) [Pubmed]
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