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Nishimura, M. et al.

Nishimura, Ishiyama, Davies,

Molecular cloning of streptomyces genes encoding vanillate demethylase.

The vanillate demethylase genes from Streptomyces sp. NL15-2K were cloned and sequenced. The vanA and vanB gene homologs, which encode the terminal oxygenase subunit (VanA) and the ferredoxin-type reductase subunit (VanB) of the enzyme respectively, were found in the sequenced 7.5-kb DNA region. Expression of the vanAB genes in Streptomyces lividans 1326 resulted in in vivo demethylation of veratric acid to vanillic acid.[1]

References

Molecular cloning of streptomyces genes encoding vanillate demethylase. Nishimura, M., Ishiyama, D., Davies, J. Biosci. Biotechnol. Biochem. (2006) [Pubmed]

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