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Zhang, Y. et al.

Yi Zhang, Guang-Ming Zeng, Lin Tang, Dan-Lian Huang, Xiao-Yun Jiang, Yao-Ning Chen,

A hydroquinone biosensor using modified core-shell magnetic nanoparticles supported on carbon paste electrode.

A hydroquinone biosensor was developed and used to determine hydroquinone concentration in compost extracts based on the immobilization of laccase on the surface of modified magnetic core-shell (Fe(3)O(4)-SiO2) nanoparticles. Laccase was covalently immobilized on the magnetic nanoparticles by glutaraldehyde, which was modified with amino groups on its surface. The obtained magnetic bio-nanoparticles were attached to the surface of carbon paste electrode with the aid of a permanent magnet to determine hydroquinone. A good microenvironment for retaining the bioactivity of laccase was provided by the immobilization matrix. The linear range for hydroquinone determination was 1 x 10(-7) to 1.375 x 10(-4)M, with a detection limit of 1.5 x 10(-8)M. The current reached 95% of the steady-state current within about 60s. Hydroquinone concentration in compost extracts was determined by laccase biosensor and HPLC, the results of the two methods were approximately the same.[1]

References

A hydroquinone biosensor using modified core-shell magnetic nanoparticles supported on carbon paste electrode. Zhang, Y., Zeng, G.M., Tang, L., Huang, D.L., Jiang, X.Y., Chen, Y.N. Biosens. Bioelectron (2007) [Pubmed]

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