Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Schilling, B. et al.

Characterization of Lipid A Acylation Patterns in Francisella tularensis, Francisella novicida, and Francisella philomiragia Using Multiple-Stage Mass Spectrometry and Matrix-Assisted Laser Desorption/Ionization on an Intermediate Vacuum Source Linear Ion Trap.

Lipopolysaccharide ( LPS) is a major component of the outer membrane of Gram-negative bacteria. The lipid A region of LPS stimulates the immune system in a structure-dependent manner. We have previously identified the two major lipid A species from Francisella tularensis as asymmetric tetraacylated structures containing four long acyl chains (16 and 18 carbons) and a single phosphate group that is partially modified by galactosamine (Phillips, N. J.; Schilling B.; McLendon, M. K.; Apicella, M. A.; Gibson, B. W. Infect. Immun. 2004, 72, 5340-5348). In the current study, we used matrix-assisted laser desorption/ionization on an intermediate vacuum source (vMALDI) coupled to a linear ion trap (LIT) mass spectrometer in multiple-stage mass fragmentation mode (MSn) to determine the structures of several minor and low abundant lipid A species present in F. tularensis, Francisella novicida, and Francisella philomiragia LPS that have not been previously characterized. Comprehensive vMALDI-MSn fragmentation studies allowed us to deduce the composition and the position of the fatty acid substituents within the lipid A moieties. Unexpectedly, most of these minor lipid A species consisted of multiple isobaric species with acyl chains of various lengths. Moreover, we found that a small portion of these lipid A species may be modified by the addition of a hexose or hexosamine sugar, in addition to the galactosamine that was previously identified. Overall, we found that MSn analysis on the vMALDI-LIT-MS platform was highly efficient and sensitive, allowing for thorough analysis of very minor lipid A species.[1]

References

Characterization of Lipid A Acylation Patterns in Francisella tularensis, Francisella novicida, and Francisella philomiragia Using Multiple-Stage Mass Spectrometry and Matrix-Assisted Laser Desorption/Ionization on an Intermediate Vacuum Source Linear Ion Trap. Schilling, B., McLendon, M.K., Phillips, N.J., Apicella, M.A., Gibson, B.W. Anal. Chem. (2007) [Pubmed]

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