The kinetics of diastereomeric amino acids with o-phthaldialdehyde.
The kinetics of the reaction of the amino acid epimers L-isoleucine, D-allo-isoleucine, L-threonine, and D-allo-threonine with o-phthaldialdehyde and mercaptoethanol were determined at 25 degrees C. L-Isoleucine reacts faster than its D-epimer whereas L-threonine reacts slightly slower than its D-epimer. In the case of isoleucine, the consequence can be an allo/iso ratio which in the worst case is 25% too low if these amino acids are quantified by liquid chromatography and o-phthaldialdehyde fluorescence detection. The effect on dating of fossils by amino acid racemization is discussed.[1]References
- The kinetics of diastereomeric amino acids with o-phthaldialdehyde. Meyer, M.W., Meyer, V.R., Ramseyer, S. Chirality. (1991) [Pubmed]
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