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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Electrophoretic human leukocyte antigen HLA-DQA1 DNA typing after polymerase chain reaction amplification.

An electrophoretic method (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is described which permits the identification of human leukocyte antigen HLA-DQA1 types and subtypes without using allele-specific oligonucleotide probes and dot-blot methodology. The procedure can be used in miniaturized gels in combination with automated electrophoretic systems. The PhastSystem is particularly recommended since temperature control is essential. HLA-DQA1*01 and DQA1*0301 can be distinguished in homoduplexes and DQA1*01 subtypes, DQA1*0201 and DQA1*0401 in heteroduplexes (in only 5 h including DNA extraction and PCR amplification). Additional variations to those recognized using commercially available dot-blot methods can be provided since this procedure permits the identification of single base-pair substitutions. In addition, this method is faster and less expensive than commercial methods.[1]

References

  1. Electrophoretic human leukocyte antigen HLA-DQA1 DNA typing after polymerase chain reaction amplification. Barros, F., Carracedo, A., Lareu, M.V., Rodriguez-Calvo, M.S. Electrophoresis (1991) [Pubmed]
 
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