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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Silver ion unusually stabilizes the structure of a parallel-motif DNA triplex.

A simple and easy method for stabilizing DNA triplexes using Ag(+) is reported. A silver ion displaces the N3 proton of cytosine in Hoogsteen base-pairing to form the base triplet CG.CAg(+). By the addition of an equimolar amount of Ag(+), the third-strand 15-mer sequence containing five cytosines was stabilized by approximately 30 degrees C in melting temperature at pH 7. The triplex structure was stable even under weakly basic conditions.[1]

References

  1. Silver ion unusually stabilizes the structure of a parallel-motif DNA triplex. Ihara, T., Ishii, T., Araki, N., Wilson, A.W., Jyo, A. J. Am. Chem. Soc. (2009) [Pubmed]
 
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