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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Detection and semiquantitative estimation of thyroxine and diiodothyronine in liothyronine sodium.

A method is presented for the detection and semiquantitative estimation of thyroxine and diiodothyronine in liothyronine sodium. Thyroxine is detected by thin layer chromatography, using silica gel H plates developed in butanol-acetone-ammonia (30 + 55 + 15) and sprayed with a 2,7-dichlorofluorescein solution, and estimated by comparison with standard spots. For quantitative results, a larger amount of sample is applied as a streak on a fluorescent silica gel H plate. After a 5 hr development, the band is scraped off and extracted in a small volume of 0.1N NaOH; the extract is centrifuged, transferred to a 2 cm microcell, and examined spectrophotometrically. The diiodothyronine content of liothyronine sodium is estimated by liquid chromatography on a Bondapak C18 column with 0.01N sodium perchloratebutanol-acetonitrile (1000 +62 + 188) as the eluting solvent.[1]

References

  1. Detection and semiquantitative estimation of thyroxine and diiodothyronine in liothyronine sodium. Cieri, U.R., Illuminati, J.C. Journal - Association of Official Analytical Chemists. (1977) [Pubmed]
 
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