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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A synthetic protein corresponding to the entire vpr gene product from the human immunodeficiency virus HIV-1 is recognized by antibodies from HIV-infected patients.

The 95 amino acid-protein encoded by the non-structural vpr gene of the human immunodeficiency virus type 1 (LAV-1BRU isolate) was chemically synthesized by solid phase methodology. The synthetic vpr protein was characterized by amino acid analysis, sequence analysis, RP-HPLC, and urea-SDS PAGE. Using a radioimmunoassay, antibodies to the synthetic protein were detected in sera of 25% of HIV 1-seropositive patients tested. Western blot analysis suggested that the antibodies preferentially recognize the dimeric form of vpr.[1]

References

  1. A synthetic protein corresponding to the entire vpr gene product from the human immunodeficiency virus HIV-1 is recognized by antibodies from HIV-infected patients. Gras-Masse, H., Ameisen, J.C., Boutillon, C., Gesquière, J.C., Vian, S., Neyrinck, J.L., Drobecq, H., Capron, A., Tartar, A. Int. J. Pept. Protein Res. (1990) [Pubmed]
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