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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A radiochemical method for the determination of transketolase activity in erythrocyte hemolysates.

A radiochemical method for the determination of transketolase activity is reported. It is based on incubation of erythrocyte hemolysates with radioactive ribose 5-phosphate followed by isolation of sedoheptulose 7-phosphate on anion-exchange columns. The experimental conditions are discussed and the presence of phosphatase activity in the incubation mixture is demonstrated. The proposed method is compared with a current colorimetric method. Reference values are given for the transketolase activity and the thiamine pyrophosphate effect.[1]

References

  1. A radiochemical method for the determination of transketolase activity in erythrocyte hemolysates. Reijnierse, G.L., van der Horst, A.R., de Kloet, K., Voorhorst, C.D. Clin. Chim. Acta (1978) [Pubmed]
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