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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit.

To facilitate functional and mechanistic studies of receptor-G protein interactions, [corrected] the human beta 2-adrenergic receptor (h beta- AR) has been expressed in Saccharomyces cerevisiae. This was achieved by placing a modified h beta- AR gene under control of the galactose-inducible GAL1 promoter. After induction by galactose, functional h beta- AR was expressed at a concentration several hundred times as great as that found in any human tissue. As determined from competitive ligand binding experiments, h beta- AR expressed in yeast displayed characteristic affinities, specificity, and stereoselectivity. Partial activation of the yeast pheromone response pathway by beta-adrenergic receptor agonists was achieved in cells coexpressing h beta- AR and a mammalian G protein (Gs) alpha subunit-demonstrating that these components can couple to each other and to downstream effectors when expressed in yeast. This in vivo reconstitution system provides a new approach for examining ligand binding and G protein coupling to cell surface receptors.[1]

References

  1. Control of yeast mating signal transduction by a mammalian beta 2-adrenergic receptor and Gs alpha subunit. King, K., Dohlman, H.G., Thorner, J., Caron, M.G., Lefkowitz, R.J. Science (1990) [Pubmed]
 
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