Fine structure of the Legionnaires' disease bacterium. In-vitro and in-vivo studies of four isolates.
We obtained four bacterial isolates from patients with Legionnaires' disease and examined them for in-vitro and in-vivo fine-structure characteristics. All isolates had an outer membrane, cytoplasmic membrane, and intracellular membrane structure. Numerous intracellular inclusions were seen, particularly from in-vivo specimens, and appeared membrane-limited. Fine-structure analysis did not reveal the presence of a definitive peptidoglycan structure. Isolation, purification, and chemical analysis of Legionnaires' disease bacterium pepdoglycan established molar ratios of alanine-glutamic acid and muramic acid-glucosamine. Diaminopimelic acid was absent in the Legionnaires' disease bacterium peptidoglycan. The Kellenberger procedure for fixation appears to be the best method for the fine-structure determination of Legionnaires' disease bacteria.[1]References
- Fine structure of the Legionnaires' disease bacterium. In-vitro and in-vivo studies of four isolates. Keel, J.A., Finnerty, W.R., Feeley, J.C. Ann. Intern. Med. (1979) [Pubmed]
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