Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

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Ohkuni, H. et al.

Hisashi Ohkuni, Hideaki Nagamune, Nana Ozaki, Atsushi Tabata, Yuko Todome, Yukino Watanabe, Hidemi Takahashi, Kazuto Ohkura, Hiroki Kourai, Hiroki Ohtsuka, Vincent A. Fischetti, John B. Zabriskie,

Characterization of recombinant Streptococcus mitis-derived human platelet aggregation factor.

We previously purified Streptococcus mitis-derived human platelet aggregation factor (Sm-hPAF) from the culture supernatant of S. mitis strain Nm-65, isolated from the tooth surface of a patient with Kawasaki disease. Here we produced recombinant Sm-hPAF protein (rSm-hPAF) in Escherichia coli, to determine whether rSm-hPAF conserves its platelet aggregation activity. rSm-hPAF precursor (665 amino acids) shows up to 36-56% identity with the family of cholesterol-dependent cytolysins (CDCs), and rSm-hPAF displayed potent hemolytic activity toward mammalian erythrocytes, including human erythrocytes with platelet aggregation activity. The 162-amino acid amino-terminal domain of rSm-hPAF was found in no other CDCs except lectinolysin; this domain is homologous to a portion of pneumococcal fucolectin-related protein. Interestingly, suilysin (SLY) and pneumolysin (PLY) of CDCs also exhibit substantial human platelet aggregation activity, similar to rSm-hPAF, and the platelet aggregation by rSm-hPAF, SLY, and PLY was morphologically confirmed using light and electron microscopy.[1]

References

Characterization of recombinant Streptococcus mitis-derived human platelet aggregation factor. Ohkuni, H., Nagamune, H., Ozaki, N., Tabata, A., Todome, Y., Watanabe, Y., Takahashi, H., Ohkura, K., Kourai, H., Ohtsuka, H., Fischetti, V.A., Zabriskie, J.B. APMIS (2012) [Pubmed]

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