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Trombley, L. et al.

Enrichment of C-type virus mRNA from immunochemically separated polyribosomes.

A method for the isolation of mRNA from a c-type virus-positive cell (JLS-V6) has been developed. This method consisted of (i) specific immunochemical precipitation of polyribosomes synthesizing viral proteins, (ii) extraction of the mRNA from the polyribosome/antigen/antibody complex, and (iii) separation of poly-A containing RNA by affinity chromatography on oligo-d(T)-cellulose. Three size classes of virus specific poly-A containing mRNA are identified and they are 35S, 20S-25S, and 10S- 15S.[1]

References

Enrichment of C-type virus mRNA from immunochemically separated polyribosomes. Trombley, L., Wang, C.S. Microbiol. Immunol. (1979) [Pubmed]

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