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Hemagglutination assay for human serum fibronectin.

A hemagglutination assay for human serum fibronectin ( Fn) is described. The assay is based on the capacity of Fn to bind to gelatin. Purified and human serum Fn agglutinate chromic chloride treated sheep red cells coated with gelatin in a quantitative and reproducible manner. The lowest Fn concentration giving positive hemagglutination was found to be 7 micrograms/ml, a sensitivity making it suitable for measurement of Fn in both normal and pathologic sera. The assay is simple and rapid and does not require specialized equipment.[1]

References

  1. Hemagglutination assay for human serum fibronectin. Bălună, R.G., Barac, D., Tarnavschi, R., Belaşcu, I. J. Immunol. Methods (1985) [Pubmed]
 
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