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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 

Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes.

Detection of viral DNA in infected tissues is demonstrated by in situ nucleic acid hybridization employing bromodeoxyuridine (BrdUrd)-labeled probes. BrdUrd is used alternatively to biotinylated nucleotides in the preparation of DNA probes and, after hybridization, developed with a monoclonal anti-BrdUrd antibody and the alkaline phosphatase-anti-alkaline phosphatase technique. The sensitivity using both BrdUrd- and biotin-labeled probes is similar. Furthermore, the simultaneous detection of viral coinfections will be possible by means of this technique in conjunction with biotin-labeled DNA probes.[1]

References

  1. Detection of viral DNA by in situ hybridization using bromodeoxyuridine-labeled DNA probes. Niedobitek, G., Finn, T., Herbst, H., Bornhöft, G., Gerdes, J., Stein, H. Am. J. Pathol. (1988) [Pubmed]
 
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