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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells.

Strontium ion formed DNA-phosphate precipitates analogous to those formed by calcium but lacking the lethal and differentiation-inducing effects of calcium on many epithelial cell types in primary culture. Human primary bronchial epithelial cells were transiently and stably transfected by using strontium phosphate; the frequency of stable transformation with a plasmid carrying the simian virus 40 large-T-antigen gene was greater than 10(-4).[1]

References

  1. Strontium phosphate transfection of human cells in primary culture: stable expression of the simian virus 40 large-T-antigen gene in primary human bronchial epithelial cells. Brash, D.E., Reddel, R.R., Quanrud, M., Yang, K., Farrell, M.P., Harris, C.C. Mol. Cell. Biol. (1987) [Pubmed]
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