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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Rapid isolation of lysyl-tRNA synthetase from rat liver.

The high molecular weight aminoacyl-tRNA synthetase complex (the 24S complex) was isolated from rat liver by ultracentrifugation. The lysyl-tRNA synthetase (E.C. 6.1.1.6) was selectively dissociated by hydrophobic interaction chromatography on 1,6 diaminohexyl agarose followed by hydroxylapatite chromatography and DEAE chromatography. The lysyl-tRNA synthetase dissociated from the 24S synthetase complex was purified approximately to 2700 fold with 14% yield.[1]

References

  1. Rapid isolation of lysyl-tRNA synthetase from rat liver. Kumar, A.M., Nayak, R. Biochem. Biophys. Res. Commun. (1988) [Pubmed]
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