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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Localization of phospholamban in smooth muscle using immunogold electron microscopy.

Phospholamban, the putative regulator of the Ca2+-ATPase in cardiac sarcoplasmic reticulum, was immunolocalized in canine visceral and vascular smooth muscle. Gently disrupted tissues were labeled with an affinity-purified phospholamban polyclonal antibody and indirect immunogold, using preembedding techniques. The sarcoplasmic reticulum of smooth muscle cells was specifically labeled with patches of immunogold distributed in a nonuniform fashion, while the sarcolemma did not appear to contain any phospholamban. The outer nuclear envelopes were also observed to be heavily labeled with the affinity-purified phospholamban polyclonal antibody. These findings suggest that phospholamban may play a role in the regulation of cytoplasmic and intranuclear calcium levels in smooth muscle cells.[1]

References

  1. Localization of phospholamban in smooth muscle using immunogold electron microscopy. Ferguson, D.G., Young, E.F., Raeymaekers, L., Kranias, E.G. J. Cell Biol. (1988) [Pubmed]
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