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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Construction of a urocanic acid-producing strain of Serratia marcescens by transduction.

In Serratia marcescens, the mutation responsible for triazolealanine (TRA) resistance was transferred from a TRA-resistant mutant to a urocanase-less mutant by PS20-mediated transduction. The two crosses were performed using as donors two TRA-resistant mutants, whose phenotypes included increased levels of histidine-biosynthetic enzymes and feedback-insensitive phosphoribosyltransferase. In one cross, TRA-resistant transductants were urocanase-less mutants having only increased levels of the enzymes and barely detectable levels of urocanic acid. In the other cross, the transductants were urocanase-less mutants having both phenotypes of the donor, and most produced high concentrations (10.5 mg/ml) of urocanic acid.[1]

References

  1. Construction of a urocanic acid-producing strain of Serratia marcescens by transduction. Kisumi, M., Nakanishi, N., Takagi, T., Chibata, I. Appl. Environ. Microbiol. (1978) [Pubmed]
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