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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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An enzyme-linked immunosorbent assay for the detection and quantitation of anti-Jo-1 antibody in human serum.

We have developed an enzyme-linked immunosorbent assay (ELISA) specific for autoantibodies directed against the autoantigen Jo-1 (histidyl-tRNA synthetase) using antigen prepared biochemically from HeLa cells. No other patient sera, including those containing antibodies directed at threonyl-tRNA synthetase or alanyl-tRNA synthetase, reacted in the assay. Screening of sera from 169 patients with a variety of autoimmune and neuromuscular diseases confirmed that anti-Jo-1 antibodies are confined to a subgroup of patients with pure polymyositis, pure dermatomyositis, or myositis associated with another rheumatic disease.[1]

References

  1. An enzyme-linked immunosorbent assay for the detection and quantitation of anti-Jo-1 antibody in human serum. Biswas, T., Miller, F.W., Takagaki, Y., Plotz, P.H. J. Immunol. Methods (1987) [Pubmed]
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