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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage.

The Escherichia coli DNA replication origin (oriC) and the adjacent asparagine synthetase gene (asnA) have been inserted into the duplex replicative form DNA of the single-stranded phage vector M13Goril. By in vitro recombination, the entire oriC asnA-containing plasmid pJS5 was inserted into M13Gori1 in both possible orientations. Both phage types transduce the asnA gene and confer upon the M13 vector the ability to replicate as a plasmid in the E. coli mutant rep3. In rep+ hosts, these phages undergo single-stranded DNA synthesis and viral morphogenesis.[1]

References

  1. Cloning and expression of the Escherichia coli replication origin in a single-stranded DNA phage. Kaguni, J., LaVerne, L.S., Ray, D.S. Proc. Natl. Acad. Sci. U.S.A. (1979) [Pubmed]
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