Biochemical characterization of the cholesterol-dependent growth of the NS-1 mouse myeloma cell line.
The biochemical basis for the cholesterol-dependent growth phenotype of the NS-1 myeloma cell line has been investigated. In one series of experiments, the growth response of NS-1 cells to several of the intermediates of cholesterol biosynthesis was studied in serum-free medium. The cholesterol precursors, squalene and lanosterol, were totally ineffective in promoting NS-1 cell growth. In contrast, cholesterol precursors downstream from lanosterol, i.e., desmosterol and 7-dehydrocholesterol, completely replaced cholesterol in supporting NS-1 cell growth. In a second series of experiments, NS-1 cells and NS-1-503 cells (a cholesterol growth-independent variant of NS-1 cells) were labelled with [2-14C]acetate and the distributions of radioactivity between cholesterol and its precursors were determined by thin-layer chromatography using two different solvent systems. The major labelled sterol product (greater than 80%) in NS-1 cells after a 24-h exposure to [2-14C]acetate was lanosterol. In contrast, the major labelled sterol product (greater than 95%) in NS-1-503 cells after a 24-h exposure to [2-14C]acetate was cholesterol. Taken together, these results indicate that NS-1 cells are defective in cholesterol biosynthesis and identify the site of lesion as the demethylation of lanosterol to C-29 sterol intermediates.[1]References
- Biochemical characterization of the cholesterol-dependent growth of the NS-1 mouse myeloma cell line. Chen, J.K., Okamoto, T., Sato, J.D., Sato, G.H., McClure, D.B. Exp. Cell Res. (1986) [Pubmed]
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